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Can I store the completed ligation reactions and transformed cells?

April 26, 2017cells ligation reactions store transformed

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Can I store the completed ligation reactions and transformed cells?

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10 Posted

Yes. After the ligation reaction has been denatured at 72° C for 15 minutes, it can be stored at -20° C indefinitely.The transformed cells that are left over after plating can be stored by adding sterile glycerol to a final concentration of 15%. Store the cells at -80°C.