Can I run a gradient with my Daicel chiral column?
In most cases, running a gradient with a Daicel chiral column is counterproductive. The time required to re-equilibrate the column back to initial mobile phase conditions is typically longer than any time-savings realized from the use of a gradient. There have been reports from some users that they find gradients useful in method development, although in general the gradients are best run over relatively limited range to avoid the long equilibration times. This avoids the situation where either the solutes elute immediately or not at all. This may be an application-dependent phenomenon where a particular class of compounds lends itself better to gradient elution than others.
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