Can I reclone the insert sequences from the SureSilencing™ shRNA plasmids into a viral-based vector?
The SureSilencing™ shRNA plasmids cannot be directly used for viral delivery, nor do they have convenient restriction sites to reclone the U1-based shRNA expression cassette into another vector system. Inserts for shRNA are more easily synthesized as oligos and then cloned into an appropriate expression system. So instead, we recommend screening the set of four shRNA sequences in another model system amenable to transfection to find the best sequence. Then, use the shRNA insert sequence information provided with the purchase of our plasmids to synthesize an oligo for cloning into your viral-based delivery system of choice.
Related Questions
- How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
- Can I reclone the insert sequences from the SureSilencing™ shRNA plasmids into a viral-based vector?
- How do I download the Users Manual for the SureSilencing™ shRNA plasmids?
