Can I perform a batch purification using the amylose resin?
Yes, batch purification works well, although it is difficult to wash all the non-specific proteins away as effectively as in a column due to the included volume in the resin. The resin can withstand centrifugation at up to 6000 x g. A good compromise is to load the resin in a batch mode, by incubating with shaking for 2 h to overnight, then pour it in a column to wash and elute. Dilution of the crude extract is not as critical for loading the column by the batch method.
