Can I modify Primerfox to calculate the annealing temperature more accurately considering more parameters?
PRIMERFOX is kept designedly very simple. So it is hold transparent much as possible and quick to use, because you need to enter only very few paramters. The resulting primers sufficient for most PCR applications. There are many other online primer designer. Most of them are much more accurate in calculating annealing temperature based on many more parameters. But that means, that you have to define many more paramters and often you can’t find primer because parameter ranges are too specific. So PRIMERFOX was created. PRIMERFOX DOES NOT REPLACE THINKING WHILE YOU DESIGN YOUR PRIMER, BUT IT SAVES PRETTY MUCH TIME AND MAKES YOU FEELING GOOD BECAUSE YOU COMPREHEND HOW IT WORKS. Nevertheless there is a way to combine primer of different PRIMERFOX queries. Just switch to the list view on the result page and copy the lists of forward and reverse primer to MS Excel (e.g.). Now you can easily calculate the PCR product length by the formula L = position(reverse primer) – position(forward primer
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