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Can I measure phase II metabolism using microsomes or S9 fractions?

April 26, 2017fractions ii measure metabolism microsomes phase s9

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Can I measure phase II metabolism using microsomes or S9 fractions?

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Yes, but with microsomes and S9 you must add the necessary phase II cofactors. For example, if you want to measure glucuronidation (UDPGT) in a microsomal incubation, you will need to add uridine diphosphoglucuronic acid (UDPGA) in addition to the normally required NADPH regenerating system.