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Can I make a stably transformed cell line using pNEBR-R1?

April 26, 2017CELL line pnebr-r1 stably transformed

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Can I make a stably transformed cell line using pNEBR-R1?

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pNEBR-R1 has a neomycin resistance marker, allowing selection of stable transformants using G418. Either linear or supercoiled pNEBR-R1 plasmid may be used to generate stable lines. Care should be taken to avoid linearizing pNEBR-R1 in such a way as to separate receptors from their promoters.