Can I have some details about Ion –exchange chromatography?
Ion Exchange Chromatography relies on charge-charge interactions between the proteins in a selected sample and the charges immobilized on the resin taken. Ion exchange chromatography can be subdivided into anion exchange chromatography, in which the negatively charged ions bind to positively charged ions whereas vise versa in cation exchange chromatography. In living things, two third of the dry matter contains protein. Hence, efforts are being made to purify a protein of interest from other proteins in a cell. In order to separate any of the macromolecules the knowledge about the chemistry of proteins like molecular weight, its charge, and its shape is required. I use www.examville.com for this kinds of problems really helping me to improve my score.
