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Can I extract the vector directly from the lentiviral particle?

April 26, 2017directly Extract lentiviral particle vector

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Can I extract the vector directly from the lentiviral particle?

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No, this is not possible. The vector is used to make the particles in the packaging or producer cells. The viral genome contains only the RNA version of the region found between the 5′ and 3′ LTRs of pLKO.1 (promoter, hairpin sequence, puromycin resistance gene, etc.