Can I estimate DNA yield using a UV spectrophotometer?
Plasmid DNA yield often is over-estimated from its OD values at 260 nm due to the contamination of protein, RNA, and RNase A generated nucleotides. Unlike DNA isolated with other kits where RNase A is used to degrade cellular RNA, AquaPlasmid isolated DNA is ultra pure and can be accurately measured with a UV spectrophotometer.
