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Can I do real-time qPCR with Phusion Blood Direct PCR Kit if I add SYBR Green to the reaction?

April 26, 2017blood kit PCR qPCR real-time

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Can I do real-time qPCR with Phusion Blood Direct PCR Kit if I add SYBR Green to the reaction?

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Phusion Blood Direct PCR Kit is optimized only for end-point PCR, not for quantitative analysis. The blood present in the PCR reaction may interfere with fluorescence detection and decrease the amplification efficiency.