Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can I do immunostaining with cells encapsulated in Extracel hydrogels?

April 26, 2017cells encapsulated hydrogels immunostaining

0

Posted

Can I do immunostaining with cells encapsulated in Extracel hydrogels?

1 Answer

0

Posted

No. Antibodies are too big to permeate the Extracel hydrogels. Antibody staining can only be done by treating the “hydrogel + encapsulated cells” as tissue. The “hydrogel + cells” can be paraffin embedded, sectioned and stained. The other alternative is to recover the cells from the hydrogel and then stain them normally.