Can I dilute the cDNAs in the RACE panel and use them for multiple experiments?
Two cDNA concentrations are provided to facilitate analysis of both abundant and rare transcripts. They are designated 5X for analysis of most transcripts and 1X for highly-abundant transcripts. These concentrations have been tested extensively to give a positive signal even for very rare transcripts (for example, 2 copies per million). In general, RACE analysis with a higher concentration of cDNAs gives you more consistent results. If the cDNA concentration is too low, or your primer specificity is not ideal, you may not be able to amplify specific but rare targets and may detect only nonspecific but abundant targets. We recommend that you use the concentration provided. If you know that your RNA is very abundant in a specific tissue, we suggest that you start by using the 1X concentration.
