Can I dilute Bactosomes before aliquotting and refreezing?
We recommend storing Bactosomes with as high a protein concentration as possible, however, we have diluted Bactosomes in storage buffer (50 mM Tris-acetate pH 7.6, 250 mM sucrose, 0.25 mM EDTA) and refrozen them and found no difference in their activity after storage at -80°C. Problems have been encountered when the Bactosomes have been diluted in buffers other than the storage buffer specified.
