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Can I determine NI susceptibility by sequencing the NA gene?

April 26, 2017determine gene Na Ni sequencing susceptibility

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Can I determine NI susceptibility by sequencing the NA gene?

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Not entirely. NI resistance is drug and influenza subtype specific. Whilst we do know some common mutations in the NA which cause resistance (H274Y in H1N1; E119V I222V orR292K in H3N2; R152K, D198E or I222T in Flu B all given in N2 numbering) the mechanism is not fully understood and there may be more not yet characterised. Sequencing can and should be used alongside phenotypic testing (enzyme inhibition tests) to characterise isolates with high IC50 values i.e. reduced NI susceptibility.