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Can I correlate functional data obtained from an individual living cell with post-fixation data obtained from the same cell?

April 26, 2017CELL correlate Data Functional individual Living obtained

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Can I correlate functional data obtained from an individual living cell with post-fixation data obtained from the same cell?

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Yes. After completing your experiments on living cells, flow fixative solution through the slide to fix the cells. Perform the fixed cell assay (e.g. immunostaining of intracellular targets).