Can I clone into pMAL vectors just as I would into pUC vectors, i.e. plating my transformants on Xgal/IPTG plates and picking colonies that are white?
No. Even though the b-galactosidase a fragment is the same on the pUC and pMAL vectors, the tac promoter on the pMAL vectors is much stronger than the lac promoter on the pUC vectors. If cells bearing a pMAL vector are induced with IPTG, the cells eventually die. The blue-to-white screen is done by replica plating (or picking and stabbing) onto a master amp plate and an amp Xgal plate containing 0.1 mM IPTG.
Related Questions
- Can I clone into pMAL vectors just as I would into pUC vectors, i.e. plating my transformants on Xgal/IPTG plates and picking colonies that are white?
- Which strand of DNA is packaged when cells carrying the pMAL vectors are superinfected with an M13 helper phage such as M13KO7 (NEB cat #315)?
- How can I obtain the sequences of the pMAL vectors?
