Can I change the needle on a calibrated syringe and maintain the calibration?
SGE syringes are calibrated using the internal dimension of the barrel, rather than measuring the volume that the syringe delivers. SGE syringes are therefore calibrated to contain, not calibrated to deliver. Changing the needle won’t change the volume contained in the syringe barrel, however it may change the amount delivered. To determine any effect of a needle change on the delivered volume, you should measure the delivered volume gravimetrically, or with a spectrophotometer.
