Can I carry out intracellular immunolabeling with WETSEM® technology?
Yes. Colloidal gold particles are well visualized with WETSEM® technology and intracellular antigens can be labeled on fixed, permeabilized cells using commercially available gold conjugates. Generally, smaller gold particles (less than 10 nm) are better suited for intracellular labeling. Particles less than 10 nm need to be Silver enhanced for imaging.
