Can human whole choromosome paints be used in immunolabelling of proteins?
• Which paint is best used to distinguish between human and mouse cells? We recommend our Star*FISH Human Chromosome-specific 22 paint. This will pick out chromosome 22, which mice do not have. Alternatively, human and mouse centromerics should not cross-react, due to the divergence between species of the repetitive sequences. Otherwise, mouse and human Y probes do not cross react (you have to increase the hybridisation time from overnight to about 3 days to get cross-reactivity). However, if you’re not always using male cells, then this option is not suitable. • When carrying out washing steps prior to detecting paints, can I use the same coplin jar of detergent wash solution? No, you should use a fresh detergent wash solution each time.
