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Can HRP or AP conjugated secondary antibodies be used for epitope analysis?

April 26, 2017analysis antibodies AP conjugated epitope hrp Secondary Used

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Can HRP or AP conjugated secondary antibodies be used for epitope analysis?

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We do not recommend the use of HRP or AP enzymes for colorimetric detection. The reaction products of these enzymes cannot be removed effectively from the membrane when regenerated according to the regeneration protocol. However, HRP and AP enzymes can be used with chemiluminescent detection protocols for analysis of the SPOTs membrane.