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Can every gene be identified in the MPSS analysis? What are the technical biases that may affect the analysis of a particular gene?

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Can every gene be identified in the MPSS analysis? What are the technical biases that may affect the analysis of a particular gene?

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Several problems can prevent identification of a specific gene in the libraries. These are all sequence-specific and so only affect a small percentage of the total genes. However, if your gene is one of those that are affected, you’re out of luck.

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