Can/do cells interact with one another in the array?
Secretagogues from one cell can diffuse freely to nearby cells. In this way cells can interact with neighboring cells, even if they are not in direct contact. Non-adherent cells, loaded into an array with picowells the size of a single cell, will not be able to make direct contact with adjacent cells. If direct contact is required, we suggest using a slide with a larger picowell diameter. Some adherent cells, loaded into an array with picowells the size of a single cell, extend projections and are thus able to make direct contact with neighboring cells. This property is highly dependent on cell type, and therefore must be tested by the researcher.
