Can detergents or other buffer systems be used?
Yes. High salt, reducing reagents like mercaptoethanol, chelating reagents, and detergents are allowed but not necessary. However, the pH has to be above pH 7.0. • Which reagents are compatible with the Strep-tag®/Strep-Tactin® interaction? Reagent Concentration Reduction Agents DTT β-mercaptoethanol Non-Ionic Detergents C8E4; Octyltetraoxyethylene max. 0.88 % C10E5; Decylpentaoxyethylene 0.12 % C10E6 0.03 % C12E8 0.005 % C12E9; Dodecyl nonaoxyethylene (Thesit) 0.023 % DM; Decyl-β-D-maltoside 0.35 % LM; N-dodecyl-β-D-maltoside 0.007 % NG; N-nonyl-β-D-glucopyranoside 0.2 % OG; N-octyl-β-D-glucopyranoside 2.34 % TX; Triton X-100 2 % Tween 20 2 % Ionic Detergents N-lauryl-sarcosine 2 % 8-HESO;N-octyl-2-hydroxy-ethylsulfoxide 1.32 % SDS; Sodium-N-dodecyl sulfate 0.1 % Zwitter-Ionic Detergents CHAPS 0.1 % DDAO; N-decyl-N,N-dimethylamine-N-oxide 0.034 % LDAO; N-dodecyl-N,N-dimethylamine-N-oxide 0.13 % Others Others Ammonium sulfate (NH4)2SO4 2 M CaCl2 max. 1 M EDTA 50 mM Ethanol 10 % Guanidi
