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CAN CUSTOMARRAY™ BE RE-USED AFTER THE FIRST HYBRIDIZATION?

April 26, 2017hybridization re-used

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CAN CUSTOMARRAY™ BE RE-USED AFTER THE FIRST HYBRIDIZATION?

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A single CustomArray™ microarray can be stripped three times using the CombiMatrix CustomArray™ Stripping Kit. The CustomArray™ Stripping protocol is based on chemical denaturation of DNA:DNA and DNA:RNA hybrids between oligonucleotide probes on microarrays and corresponding labeled targets. As a result of this denaturation, labeled targets are removed from oligonucleotide probes and washed off microarrays. The resultant stripped CustomArray™ microarrays can be used in the same way as newly synthesized ones. In total, the CombiMatrix Stripping Kit would enable you to use a single CustomArray™ microarray four times.