Can ColCount be used to count 3-D colonies or spheroids grown in soft agar or methylcellulose substrates?
While designed to count stained colonies grown on the surface of culture dishes, ColCount has been successfully used to count unstained colonies grown in 3-D such as agar or methylcellulose matrices. However, ColCount is NOT a microscope and for best results, these colonies/spheroids should be sufficiently large to be just visible to the naked eye. Because ColCount reads 6- well plates upside down, it will not work for colonies in soft matrices that have been grown in 6-well plates.
