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Can Bovine Serum Albumin (BSA) be replaced by other things such as Fetal Bovine Serum (FBS), in the ELISA buffers?

April 26, 2017albumin Bovine BSA Buffers ELISA fbs Fetal serum

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Can Bovine Serum Albumin (BSA) be replaced by other things such as Fetal Bovine Serum (FBS), in the ELISA buffers?

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Yes, the 1% BSA may be substituted with 10% FBS for plate blocking. Dilution of the standard, serum samples and detection antibody, in buffers containing 10% FBS may be preferable for testing of serum samples or tissue culture fluids.