Can BD Cytofix/Cytoperm buffers be used in BD Phosflow applications?
No, BD Cytofix/Cytoperm™ buffers were developed for intracellular cytokine staining and BD Phosflow antibodies have been specifically tested for use with the BD Phosflow buffers. BD Perm/Wash™ Buffer (Cat. No. 554723) included in BD Cytofix/Cytoperm™ kit (554714) has not been shown to permeabilize the nucleus, so we recommend using BD Phosflow perm buffers. BD Cytofix™ fixation buffer (Cat. No. 554655) can be used for fixation in Protocols II or III when lysis of RBCs is not required. If RBC lysis is required, the use of BD Phosflow Lyse/Fix Buffer (Cat. No. 558049) is recommended.
