Can antibiotics be used in media during transfection?
We discourage using antibiotics during transfection. Higher cell death can occur when antibiotics are present during transfection. Even though penicillin and streptomycin are not toxic to eukaryotic cells, during transfection the cell permeability increases so that much higher levels of antibiotics get into cells. For stable transfections, wait at least 72 h after transfection before adding selective antibiotics.
