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Can AmpliTaq Gold™ be used in a one-step or a two-step RT-PCR?

April 26, 2017amplitaq one-step rt-pcr two-step Used

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Can AmpliTaq Gold™ be used in a one-step or a two-step RT-PCR?

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Yes. ABI now sell the GeneAmp® Gold RNA PCR Reagent kit (P/N 4308206), which comes with a pAW109 kit control, as well as the GeneAmp® Gold RNA PCR Core Kit (P/N 4312765) that does not contain the control reagents. If you would like more information as to how it can be used in either a one-step or two-step RT-PCR reaction, you can get a copy of the kit protocol. For ordering information, please refer to the Applied Biosystems electronic store.