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Can all high density growth media reach OD600 = 30 to 50?

April 26, 2017density growth high media od600 REACH

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Can all high density growth media reach OD600 = 30 to 50?

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Yes. All these high density growth media can reach OD600 = 30 to 50 in a shake flask container. Most wild-type cell strains can reach OD600 = 20 to 30 in SecProTM medium. Some cell strains with some toxic proteins do not grow well in SecProTM medium. In these cases, the cells may be grown in DetoXTM or ProGroTM medium to reach inducible OD and induce the cells in SecProTM medium to get high yield secretary expression.