Can a combination of glutathione and granulocyte –colony stimulating factor increase chemoprotection?
5-FU is a chemotherapeutic agent with myelosuppressive effects, glutathione has been proved to have aprotective action against this myelosuppression. Also granulocyte colony stimulating factor is well known to be a protective agent aginst chemotherapeutics induced bone marrow depression. Whether glutathione (GSH) and granulocyte colony stimulating factor (G-CSF) combination offered more protection on myelosuppression induced by 5-fluorouracil (5-FU) or not was questioned in female mice. The animals were divided into seven groups (10 mice each) . Group 1 received no treatment , group 2 received GSH, group 3 received G-CSF, Group 4 received a single dose of 5-FU, Group 5 received GSH+5-FU, Group 6 received G-CSF + 5-FU and group 7 received daily doses of both GSH and G-CSF + 5-FU. Animals were sacrificed on day 9 i.e. 1 day after 5-FU administration and were evaluated using complete blood count. Surprisingly combination of GSH and G-CSF nullified the protective effects of each other.
