Can a certain concentration of antibody be recommended for labeling of cells with a primary antibody?
There is no defined antibody concentration that can generally be recommended for labeling of cells with a primary antibody. Each suitable antibody concentration depends on the target antigen expression on the cell surface, as well as on the affinity of the individual antibody. Therefore, the ideal concentration of a primary antibody has to be determined experimentally.
Related Questions
- The isotype of my primary antibody will not fix complement. Can I use a secondary antibody that fixes complement to deplete cells labeled with this primary antibody?
- How can the ideal concentration of an antibody for primary labeling be determined?
- What are the recommended primary antibody incubation times and temperatures?
