Can 4-thiodU be used for crosslinking and how is it prepared?
RESPONSE:4-thioU is efficiently activated for crosslinking by exposure to long-wavelength UV light for up to 10 minutes. Crosslinks are formed with RNA and proteins. Oligonucleotides containing 4-thiodU (10-1051) and 4-thioT (10-1033) can be produced from the triazole modified phosphoramidites using the convertible nucleoside strategy (1,2). However, we now offer the nuclesides 4-thio-dT (10-1034), 4-thio-dU (10-1052), 2-thio-dT (10-1036), and 4-thio-U (10-3052) for direct incorporation into oligonucleotides and the convertible monomers have been discontinued. A further enhancement of this strategy used 4-thioxU as an intermediated in the preparation of thiocarbonyl crosslinkers (3). REFERENCE(S): (1) Y.Z. Xu, Q. Zheng, and P.F. Swann, J. Org. Chem., 1992, 57, 3841. (2) The Glen Report, 1993, 6.1, 1, and references cited therein. (3) R.S. Coleman and J.M. Siedlecki, Journal of the American Chemical Society, 1992, 114, 9229-9230. FAQs QUESTION: What methods are available for crosslinkin
