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BrainBits neurons are growing more as clusters or clumps of cells instead of isolated. Whats wrong?

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BrainBits neurons are growing more as clusters or clumps of cells instead of isolated. Whats wrong?

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This means that adhesion of cells to themselves is tighter than to the substrate. The most likely problem is a poorly prepared substrate. Compare your protocol to the recommended preparation of polylysine and coating of substrates.

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