At which steps in the TargetAmp RNA amplification process can I stop?
The stopping points are following the 2nd-strand cDNA synthesis steps, after DNase I treatment of the in vitro transcribed aRNA (just prior to the aRNA purification steps) and after the aRNA purification steps. If you choose to stop at the cDNA step you could save your sample at -20°C. We typically store our aRNA samples at -80°C.
Related Questions
- EPICENTRE claims that the TargetAmp RNA amplification process is a "simplified and improved" Eberwine RNA amplification procedure. What have you done?
- Is it possible to scale up or scale down the TargetAmp RNA amplification reaction?
- At which steps in the TargetAmp RNA amplification process can I stop?
