assay compared to an assay that measures e.g. caspase‑3 activity?
Both assays measure different processes during apoptosis. The caspase‑3 assay measures enzyme activity (with all the limited specificity problems “peptide caspase”‑substrates have), while the M30‑Apoptosense assay does measure the accumulation of a caspase cleavage product (CK18 with the limitation to work only with epithelial cells). Due to the accumulation of the analyte (CK18Asp396‑NE M30 neo‑epitope) over time, M30‑Apoptosense is more sensitive and without the need to take into consideration the optimal analytical (often transient) window. It makes this assay convenient and economical when a large number of samples are analyzed. Caspase‑3 activity assays can only be used with samples from cell extracts, while the M30‑Apoptosense assay can be used with culture supernatants and human serum or plasma samples. Q7: I need an apoptosis specific assay that is sensitive and robust. A: The M30‑Apoptosense assay is very sensitive, as it easily detects apoptosis in less than 5,000 carcinoma c
