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assay be used for?

April 26, 2017assay Used

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assay be used for?

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The M30‑Apoptosense is an ELISA for the quantitative detection of the apoptosis‑associated M30 neo‑epitope (CK18Asp396‑NE) in cell extracts, cell culture supernatants, and in human serum or plasma samples given the cells express CK18 (are of epithelial origin). Potential applications e.g. include HTS screening for apoptotic drug candidates, performing time course kinetics, measuring dose responses after exposure to pro‑apoptotic agents. Q4: Briefly describe the procedure for the M30‑Apoptosense assay. A: The assay is a solid‑phase, two‑site immunosorbent assay (ELISA). Samples (25 l) containing caspase‑cleaved CK18 (CK18Asp396‑NE: M30 neo‑epitope) added into the wells bind to an immobilized monoclonal catcher antibody specific to CK18 and are subsequently detected with the CK18Asp396‑NE‑specific M30 monoclonal antibody conjugated to HRP as a tracer. After the formation of the solid phase/antigen/labeled antibody sandwich, excess unbound tracer is removed by washing. TMB substrate is th