Are there variations in processing times for some tissue types?
Yes, when dealing with bone and brain tissue. In the case of bone, the high density of the decalcified bone requires an increase in wax impregnation times, by as much as 50%, to compensate for the increased resistance to wax penetration. In the case of brain tissue, the high density of the lipid content requires extended extraction time for clearing of these lipids, by as much as 50%. The need for an increase in time also applies in the case of conventional processing where longer processing times are usually required, especially for the larger blocks. In both brain and bone, the need for longer times will depend on the size of the samples.
