Are there specific recommendations to prevent RNA degradation by RNases when isolating RNA with the FastRNA Pro Kits?
The origin of RNA degradation is often action of RNases: both endogenous and exogenous RNases. 1. Inactivation of endogenous RNases Endogenous RNases are released from cellular compartments immediately after harvesting tissue and cells. It is essential to inactivate these RNases as soon as possible to prevent RNA degradation. To effectively inactivate endogenous RNases, add RNApro Solution (chaotropic-based cell lysis solution containing guanidium isothiocyanate) to each sample as soon as possible following sample harvest and homogenize immediately with the FastPrep®-24 instrument or flash-freeze samples in liquid nitrogen. To prevent RNA degradation, it is important that the tissue be cut in small enough (1cm) pieces to allow rapid, thorough freezing of the entire tissue. 2. Reduce exposure to exogenous RNases To isolate intact, high quality RNA, it is essential that exogenous RNases are not introduced into purified RNA preparations. It is essential that any item that could contact th
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