Are there specific guidelines for samples with GFP?
Material containing green fluorescent protein (GFP) can be imaged without any difficulty. Typically, fixed samples have lower levels of fluorescence as compared to live cells. If at all possible, GFP should be imaged in live material. The choice of mounting medium can greatly affect the fluorescence levels. Especially important is to avoid using nail polish to seal the coverslips. This will ruin the GFP. Instead, use rubber cement to seal the slides.
