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Are there special considerations I need to take into account when using the eFluorTM 605NC and eFluorTM 625NC conjugated antibodies?

April 26, 2017account antibodies conjugated considerations efluortm special

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Are there special considerations I need to take into account when using the eFluorTM 605NC and eFluorTM 625NC conjugated antibodies?

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• Staining buffer – For the brightest staining intensity we recommend the use of the eFluorTM NC Flow Cytometry Staining Buffer (cat. 00-3222) instead of a PBS-based buffer or eBioscience Flow Cytometry Staining Buffer (cat. 00-4222). • Lysed Whole Blood (LWB) – Depending on the blood collection method used, you may need to modify your staining procedure. Please see the Flow Cytometry FAQ below for details. • Intracellular staining – Studies are ongoing to determine the compatibility of the eFluorTM Nanocrystal conjugated antibodies with intracellular staining. Please contact eBioscience Technical Support for further details. • Long term fixation – We do not recommend long-term fixation, but rather we recommend a fixation time of 30 minutes in 2% paraformaldehyde (PFA) in PBS followed by a wash in eFluorTM NC Flow Cytometry Staining Buffer.