Are there special considerations I need to take into account when using the eFluor 605NC, eFluor 625NC, and eFluor 650NC conjugated antibodies?
• Staining buffer – For the optimal staining intensity we recommend the use of the eFluor NC Flow Cytometry Staining Buffer (cat. 00-3222) instead of a PBS-based buffer or eBioscience Flow Cytometry Staining Buffer (cat. 00-4222). • Intracellular staining – Please see the Intracellular Staining protocol as found in the BestProtocols section for information regarding intracellular staining. • Long term fixation – We do not recommend long-term fixation, but rather we recommend a fixation time of 30 minutes in 2% formaldehyde. For fixation we recommend that cells be suspended in 100 µg of eFluor NC Flow Cytometry Staining Buffer and 100 μl of eBioscience IC Fixation Buffer cat. 00-8222, incubated for 20-30 minutes and then washed 1-2X with eFluor NC Flow Cytometry Staining Buffer (cat. 00-3222). The fixed cells should then be stored at 4°C in the dark until analyzed.
Related Questions
- Are there special considerations I need to take into account when using the eFluorTM Nanocrystal conjugated antibodies for flow cytometry?
- Are there special considerations I need to take into account when using the eFluorTM 605NC and eFluorTM 625NC conjugated antibodies?
- Can I use the eFluor 605NC, 625NC, or 650NC conjugated antibodies together in the same sample?
