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Are there rules for designing oligonucleotides?

April 26, 2017designing oligonucleotides rules

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Are there rules for designing oligonucleotides?

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ZytoVision uses its own, unpublished algorithm for the design of oligonucleotide probes for the use in in situ hybridization experiments. Generally oligonucleotides for use with ZytoFast systems should have: (a) a length of 28-32 nucleotides and (b) a GC content of 40-60%.