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Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?

applied Biosystems Default PCR probe problems quantifast setting threshold
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Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?

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No. We recommend using QuantiFast Probe +ROX Vial Kits: the master mix does not contain ROX dye, but the kit is supplied with a separate tube of ROX dye. The kits allow to adjust the ROX concentration…

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