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Are there known ways to accelerate the hydrolysis of DTT from the C-terminus of the target protein in a controlled manner?

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Are there known ways to accelerate the hydrolysis of DTT from the C-terminus of the target protein in a controlled manner?

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Storage of the eluted protein samples at higher pH – such as 8 or 9 will increase the rate of hydrolysis. Also storage at higher temperature, such as room temperature, will allow hydrolysis to proceed faster than at 4ÂșC.

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