Are there domains of higher fluorescence in various parts of individual cilia?
Previous ultrastructural experiments have suggested that the distal cilia are the main site of olfactory signal transduction, containing the molecular components of the cyclic nucleotide cascade (Menco et al., 1992; Menco, 1994). We therefore examined whether the fluorescence increase resulting from CNG channel activation showed a heterogeneous distribution in individual cilia, displaying higher intensity at distal cilia as compared with more proximal regions (Fig. 7). Potential problems in this analysis could result from the fact that the diameter of a given cilium is not constant over its length (Menco, 1994) and that the optical section of the confocal microscope used here was larger than the ciliary diameter. Thus, differences in fluorescence intensity over the length of a given cilium simply could reflect differences in the path length and the number of dye molecules. To avoid this problem, we measured the relative decrease in cGMP-induced fluorescence (100 µM 8-Br-cGMP) that occu
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