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Are there any “tips” regarding the microscope set up for the examination of an unstained wet preparation?

April 26, 2017microscope unstained wet

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Are there any “tips” regarding the microscope set up for the examination of an unstained wet preparation?

1 Answer

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10 Posted

The microscope should be aligned properly using Köhler illumination. Make sure the light is not too bright; otherwise, you may shine right through the organisms and miss the parasites. Also, you may want to close the diaphragm a bit to provide a bit more contrast. Extra contrast is particularly important when reading with saline only (no iodine added).