Are there any rules or guidelines as to what I can and cannot do with the samples in making a new ItSpace piece?
Other than the use restrictions laid out by the Creative Commons licence, such as no commercial use, there are no hard compositional rules. The nine core objects chose to make their characteristic sounds very evident in their pieces. This means that they processed their sounds but not to the degree that they lost an audible connection to the source material. Ideally, new ItSpace objects use only their own sounds and do not use accompaniment from traditional instruments like guitar, drums, piano, etc.