Are there any restrictions to the size of the amplicon in Direct PCR?
The shorter the amplicon, the easier the Direct PCR protocol. The maximum length depends on the sample type, sample size and the reaction volume. For example we have amplified a 7.5 kb fragment directly from blood samples, a 3.2 kb fragment from mouse ear tissue and a 300 bp fragment from FFPE tissues. Please see the individual protocols and experimental data on the Direct PCR page.
